Search results for "VegT mRNA"

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Specific binding of VegT mRNA localization signal to membranes in Xenopus oocytes

2021

Abstract We have studied the interaction of a VegT mRNA localization signal sequence with the membranes of the mitochondrial cloud in Xenopus oocytes, and the binding of the VegT mRNA signal sequence to the lipid raft regions of the vesicles bounded by ordered and disordered phospholipid bilayers. RNA preference for the membranes of the mitochondrial cloud was confirmed using microscopy of a fluorescence resonance energy transfer from RNA molecules to membranes. Our studies show that VegT mRNA has a higher affinity for ordered regions of lipid bilayers. This conclusion is supported by the dissociation constant measurements for RNA-liposome complex and the visualization of the FRET signal be…

Signal peptideXenopusLipid vesiclesMitochondrial cloudProtein Sorting SignalsXenopus ProteinsXenopus laevis03 medical and health sciencesMembrane MicrodomainsRafts0302 clinical medicineFluorescence Resonance Energy TransferAnimalsLipid bilayerMolecular BiologyLipid raftXenopus oocytes030304 developmental biology0303 health sciencesMessenger RNABinding SitesbiologyChemistryVegT mRNARNACell BiologyMembrane RNAbiology.organism_classificationFörster resonance energy transferLiposomesOocytesFRETBiophysicsFemaleT-Box Domain Proteins030217 neurology & neurosurgeryBiochimica et Biophysica Acta (BBA) - Molecular Cell Research

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